abstract：:We have previously reported that medium conditioned by hypoxic pulmonary artery endothelial cells (ECCM) contains a factor of small molecular weight that inhibits the growth of pulmonary artery smooth muscle cells (SMC). We postulated that this factor might be a breakdown product of ATP and, therefore, measured the le...

journal_title：American journal of respiratory cell and molecular biology

authors： Hassoun PM,Shedd AL,Lanzillo JJ,Thappa V,Landman MJ,Fanburg BL

更新日期：1992-06-01 00:00:00

abstract：:In addition to inhibiting proliferation and causing enlargement of bovine pulmonary artery endothelial cells in culture, porcine platelet transforming growth factor-beta 1 (TGF-beta 1) (2 ng/ml) lowered glutathione (GSH) of these cells by 48% after 96 h in culture when GSH levels were normalized for cell counts. This ...

journal_title：American journal of respiratory cell and molecular biology

authors： White AC,Das SK,Fanburg BL

更新日期：1992-04-01 00:00:00

abstract：:A comprehensive investigation of the morphology of human airway epithelial tight junctions was carried out by freeze-fracture electron microscopy using quantitative methods designed to analyze a range of junctional characteristics. Extrapulmonary bronchi that appeared grossly normal were taken at sites distant from tu...

journal_title：American journal of respiratory cell and molecular biology

authors： Godfrey RW,Severs NJ,Jeffery PK

更新日期：1992-04-01 00:00:00

abstract：:To identify mechanisms that allow p185HER2 expression in lung cancer, we performed Western, Southern, and Northern blot analyses of 14 cell lines derived from human non-small cell lung carcinomas and one cell line derived from a human mesothelioma. Human bronchiole epithelial cells and rat type II pneumocytes were fou...

journal_title：American journal of respiratory cell and molecular biology

authors： Kern JA,Robinson RA,Gazdar A,Torney L,Weiner DB

更新日期：1992-04-01 00:00:00

abstract：:Recent evidence from genetic experiments in yeast and from studies using guanosine triphosphate (GTP) analogues in mammalian cells suggests a key role for low-molecular-mass GTP-binding proteins (LMM-GBPs) (Mr 19 to 28 kD) in processes of intracellular vesicular sorting and secretion. Assembly and exocytosis of the la...

journal_title：American journal of respiratory cell and molecular biology

authors： Rubins JB,Panchenko M,Shannon TM,Dickey BF

更新日期：1992-03-01 00:00:00

abstract：:Endogenous hydrogen peroxide (H2O2) release from aortic endothelial cells was studied in the presence of antioxidant enzyme inhibitors, mitochondrial inhibitors, a microsomal cytochrome P-450 inhibitor, and after oxidative stress induced with H2O2 or menadione. Extracellular H2O2 generation was determined spectrofluor...

journal_title：American journal of respiratory cell and molecular biology

authors： Kinnula VL,Whorton AR,Chang LY,Crapo JD

更新日期：1992-02-01 00:00:00

abstract：:In surgically excised nasal polyps, most epithelial mast cells were formalin sensitive, chloroacetate esterase (CAE) negative, and chymase negative. Thus, this represents a population of mast cells not identified by staining for CAE. On the other hand, most stromal mast cells were formalin resistant and CAE positive, ...

journal_title：American journal of respiratory cell and molecular biology

authors： Kawabori S,Denburg JA,Schwartz LB,Irani AA,Wong D,Jordana G,Evans S,Dolovich J

更新日期：1992-01-01 00:00:00

abstract：:Pseudomonas aeruginosa colonizes the lower respiratory tracts of patients with severe bronchiectasis, including cystic fibrosis, a condition associated with increased airway mucus output. We have shown that an extract containing chloroform-soluble extracellular products of P. aeruginosa releases glycoconjugates into t...

journal_title：American journal of respiratory cell and molecular biology

authors： Somerville M,Taylor GW,Watson D,Rendell NB,Rutman A,Todd H,Davies JR,Wilson R,Cole P,Richardson PS

更新日期：1992-01-01 00:00:00

abstract：:The purpose of this study was to elucidate the effects of aging on diaphragmatic mitochondrial function. Diaphragm mitochondria were prepared from specific pathogen-free rats aged 7 wk (n = 7), 35 wk (n = 7), and 55 wk (n = 7). The activities of various portions of the mitochondrial electron transport chain, i.e., com...

journal_title：American journal of respiratory cell and molecular biology

authors： Torii K,Sugiyama S,Takagi K,Satake T,Ozawa T

更新日期：1992-01-01 00:00:00

abstract：:Normal lung structure is maintained by the presence of mesenchymal cells and their extracellular matrix products. The slow normal turnover of these cells is disrupted in fibrotic disorders, resulting in the in situ accumulation of mesenchymal cells and their extracellular matrix leading to a progressive alveolar wall ...

journal_title：American journal of respiratory cell and molecular biology

authors： Vignaud JM,Allam M,Martinet N,Pech M,Plenat F,Martinet Y

更新日期：1991-12-01 00:00:00

abstract：:Gene expression for tropoelastin, the proprotein for elastin, was examined in the rat lung from 17 days of gestation (pseudoglandular stage) to adulthood by in situ hybridization using a rat-specific 35S-radiolabeled riboprobe. The tropoelastin message was present in vascular and airway smooth muscle, endothelial, sep...

journal_title：American journal of respiratory cell and molecular biology

authors： Noguchi A,Samaha H

更新日期：1991-12-01 00:00:00

abstract：:Prior studies have demonstrated that human alveolar macrophages (AM) are suppressive of lymphocyte function, through the mechanism of inhibition is unclear. In the current study, human AM inhibited receptor-mediated increases in intracellular calcium concentration ([Ca2+]i) in T cells, natural killer cells, and B cell...

journal_title：American journal of respiratory cell and molecular biology

authors： Yarbrough WC Jr,Wilkes DS,Weissler JC

更新日期：1991-11-01 00:00:00

abstract：:The time course and nature of the cellular response to left pneumonectomy, with or without prior adrenalectomy, were evaluated in the right lungs of male Sprague-Dawley rats using morphometric techniques. Animals were studied at days 2, 5, and 14 following pneumonectomy, intervals prior to, during the course of, and f...

journal_title：American journal of respiratory cell and molecular biology

authors： Rannels DE,Stockstill B,Mercer RR,Crapo JD

更新日期：1991-10-01 00:00:00

abstract：:Surface airway epithelium is frequently injured by noxious inhaled agents, epithelial wound repair may be an important process by which the epithelial barrier integrity is maintained. To evaluate the role of surface airway cells in the wound repair process, we developed an in vitro wounding model of human nasal epithe...

journal_title：American journal of respiratory cell and molecular biology

authors： Zahm JM,Chevillard M,Puchelle E

更新日期：1991-09-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF-alpha) is an important inflammatory mediator produced by activated monocytes and macrophages. We have previously shown that porcine alveolar macrophages (PAM) mediate bystander cytotoxicity through hydrogen peroxide production following activation with immobilized IgG immune complex (I...

journal_title：American journal of respiratory cell and molecular biology

authors： Kim JW,Wierda WG,Kim YB

更新日期：1991-09-01 00:00:00

abstract：:Hypersensitivity pneumonitis (HP) is an allergic granulomatous interstitial lung disease resulting from a reaction of selected individuals to repeated inhalations of certain antigens. HP is characterized by chronic inflammation, and the development of the disease seems to be immunologically mediated. In farmer's lung,...

journal_title：American journal of respiratory cell and molecular biology

authors： Denis M,Cormier Y,Tardif J,Ghadirian E,Laviolette M

更新日期：1991-08-01 00:00:00

abstract：:The role of guanine nucleotide-binding proteins in the induction of prostacyclin synthesis by stimulated endothelial cells is incompletely understood. We report that sodium fluoride (NaF), a potent activator of cellular guanine nucleotide-binding proteins, affected time- and concentration-dependent generation of prost...

journal_title：American journal of respiratory cell and molecular biology

authors： Garcia JG,Dominguez J,English D

更新日期：1991-08-01 00:00:00

abstract：:Surfactant protein D (SP-D) is a collagenous, surfactant-associated, carbohydrate-binding protein that is synthesized by pulmonary epithelial cells. In the present studies, we examined the expression of SP-D and SP-D mRNA during late fetal (day 17, 19, 21) and early postnatal (day 5) rat lung development using immunoc...

journal_title：American journal of respiratory cell and molecular biology

authors： Crouch E,Rust K,Marienchek W,Parghi D,Chang D,Persson A

更新日期：1991-07-01 00:00:00

abstract：:The insulin-like growth factor (IGF)-II/mannose-6-phosphate (M6P) receptor, which targets acid hydrolases to lysosomes, is a multifunctional protein with separate binding sites for IGF-II and M6P. The purpose of this study was to determine if alveolar macrophages (AM) and their precursor cells, blood monocytes, expres...

journal_title：American journal of respiratory cell and molecular biology

authors： Rom WN

更新日期：1991-06-01 00:00:00

abstract：:Rat alveolar type II pneumocytes, in situ, label with Maclura pomifera agglutinin (MPA), a plant lectin that recognizes alpha-galactosyl oligosaccharide residues of glycoproteins and glycolipids. To study the glycoproteins recognized by the lectin, MPA lectin affinity chromatography was used to isolate a novel glycopr...

journal_title：American journal of respiratory cell and molecular biology

authors： Lwebuga-Mukasa JS

更新日期：1991-06-01 00:00:00

abstract：:Endothelin, an endothelium-derived vasoconstrictor peptide, has recently been reported to exist in airway epithelial cells. To elucidate a possible role of endothelin on epithelial functions, we studied the effects of this peptide on ciliary beat frequency (CBF) and electrical properties of canine cultured tracheal ep...

journal_title：American journal of respiratory cell and molecular biology

authors： Tamaoki J,Kanemura T,Sakai N,Isono K,Kobayashi K,Takizawa T

更新日期：1991-05-01 00:00:00

abstract：:We have determined eicosanoid production from endogenous arachidonic acid by neonatal lamb lungs stimulated with calcium ionophore A23187 during normoxia and hypoxia. Lungs of lambs 19 to 25 d of age were isolated and perfused with cell-free Krebs' bicarbonate buffer at a flow rate of 15 to 20 ml/kg/min. After 30 min ...

journal_title：American journal of respiratory cell and molecular biology

authors： Ibe BO,Isenberg WB,Raj JU

更新日期：1991-04-01 00:00:00

abstract：:To investigate mechanisms regulating intra-alveolar coagulation, we studied monolayers of the A549 human lung epithelial cell line. The surface of A549 cells delayed the onset of prothrombin-to-thrombin conversion and prevented total prothrombin consumption in normal plasma compared to plastic cell-free wells. Similar...

journal_title：American journal of respiratory cell and molecular biology

authors： Berry L,Andrew M,Post M,Ofosu F,O'Brodovich H

更新日期：1991-04-01 00:00:00

abstract：:We examined the ability of conditioned medium (CM) generated by human upper airway epithelial (Ep) cells from normal (NN) and inflamed, allergic rhinitis (AR) and nasal polyp (NP) tissues to induce monocytic differentiation of hemopoietic progenitors of the HL-60 myeloid leukemia cell line in vitro. In HL-60 cells cul...

journal_title：American journal of respiratory cell and molecular biology

authors： Ohtoshi T,Vancheri C,Cox G,Gauldie J,Dolovich J,Denburg JA,Jordana M

更新日期：1991-03-01 00:00:00

abstract：:Electrical field stimulation (EFS) has previously been shown to induce the release of prostaglandin (PG) E2 from ferret tracheal epithelium. We have now conducted a study to see whether this effect of EFS is due to the activation of nerves or whether it is a non-neural effect. The release of PGE2 and 6-keto-PGF1 alpha...

journal_title：American journal of respiratory cell and molecular biology

authors： Ullman A,Ciabattoni G,Svedmyr N,Skoogh BE,Löfdahl CG

更新日期：1991-03-01 00:00:00

abstract：:Influenza virus attaches primarily to ciliated cells in mature airways epithelium. This process is mediated by a viral envelope glycoprotein (hemagglutinin) that binds to sialic acid-containing receptors in the apical membrane of host cells. The purpose of this study was to determine the cellular distribution of these...

journal_title：American journal of respiratory cell and molecular biology

authors： Piazza FM,Carson JL,Hu SC,Leigh MW

更新日期：1991-01-01 00:00:00

abstract：:Cytolytic lymphocytes play an important role in defense against viral and neoplastic disease. Integral to the function of these cells is the content of lysosomal granules. Recent attention has focused on a family of proteases present in the granules of natural killer (NK) cells, interleukin-2 (IL-2)-activated NK cells...

journal_title：American journal of respiratory cell and molecular biology

authors： Breite WM,Dal Nogare AR,Yarbrough WC Jr,Weissler JC

更新日期：1990-12-01 00:00:00

abstract：:HLA-DR class II molecules are expressed by a variety of nonlymphoid cells, including the respiratory epithelium. However, it is not known if ciliated bronchial epithelial cells express the HLA-DR genes, if the expression of class II molecules on their surface can be modulated by immune mediators and, finally, if these...

journal_title：American journal of respiratory cell and molecular biology

authors： Rossi GA,Sacco O,Balbi B,Oddera S,Mattioni T,Corte G,Ravazzoni C,Allegra L

更新日期：1990-11-01 00:00:00

abstract：:We have previously characterized dog mastocytoma cells propagated in nude mice. We have established two of these lines (C1 and C2) in continuous culture. Freshly disaggregated mastocytoma cells were cultured in Dulbecco's modified Eagle's medium (DME)-H16 mixed with 50% Ham's F12 and supplemented with histidine and 5%...

journal_title：American journal of respiratory cell and molecular biology

authors： DeVinney R,Gold WM

更新日期：1990-11-01 00:00:00

abstract：:The cellular events involved in generating the hypoxic pulmonary vasoconstriction response are not clearly understood, in part because of the multitude of factors that alter pulmonary vascular tone. The goal of the present studies was to determine if a cell culture preparation containing vascular smooth muscle (VSM) c...

journal_title：American journal of respiratory cell and molecular biology

authors： Murray TR,Chen L,Marshall BE,Macarak EJ

更新日期：1990-11-01 00:00:00

abstract：:While elastin degradation is a hallmark of pulmonary emphysema, it is likely that elastin synthesis also occurs. However, the supramolecular structure and function of the newly synthesized elastin are abnormal. Very little is known about the regulation of elastin synthesis during the development of emphysema when prom...

journal_title：American journal of respiratory cell and molecular biology

authors： McGowan SE,McNamer R

更新日期：1990-10-01 00:00:00

abstract：:The differentiated phenotype of the alveolar type II cell is rapidly altered in vitro. To evaluate factors that might influence this process, we isolated and plated rat type II cells in serum-supplemented media to promote adherence and then maintained the cells in a simple nutrient medium in the absence (S- cells) or ...

journal_title：American journal of respiratory cell and molecular biology

authors： Paine R 3rd,Joyce-Brady M,Clement A,Brody JS

更新日期：1990-10-01 00:00:00

abstract：:Platelet-activating factor (PAF) is a potent contractile agonist in guinea pig peripheral lung strips. Whether PAF acts via specific receptor sites in the lung has not been fully established. To determine if specific receptor sites could be demonstrated in guinea pig peripheral lung tissue, we performed direct radioli...

journal_title：American journal of respiratory cell and molecular biology

authors： Gomez J,Bloom JW,Yamamura HI,Halonen M

更新日期：1990-09-01 00:00:00

abstract：:T lymphocytes and alveolar macrophages accumulating in the lower respiratory tract of patients with pulmonary sarcoidosis are known to be activated to produce several cytokines, presumably leading to granuloma formation within the lung. We hypothesized that these cells produce colony-stimulating factors (CSFs), which ...

journal_title：American journal of respiratory cell and molecular biology

authors： Itoh A,Yamaguchi E,Kuzumaki N,Okazaki N,Furuya K,Abe S,Kawakami Y

更新日期：1990-09-01 00:00:00

abstract：:Quantitative measurement of [3H]thymidine [( 3H]TdR) incorporation into cultured cells is widely used as an indicator of cell proliferation. The observation that adult type II cells are able to incorporate large amounts of [3H]TdR despite the fact that they are not proliferating raised the question of the meaning of [...

journal_title：American journal of respiratory cell and molecular biology

authors： Clement A,Riedel N,Brody JS

更新日期：1990-08-01 00:00:00

abstract：:Techniques are described for visualizing intracellular tropoelastin at the light level using immunofluorescence and immunogold techniques. Best results were obtained with B5 fixative on cells permeabilized with acetone. Using either formaldehyde or paraformaldehyde for fixation (instead of B5) resulted in both less re...

journal_title：American journal of respiratory cell and molecular biology

authors： Grosso LE,Whitehouse LA,Mecham RP

更新日期：1990-07-01 00:00:00

abstract：:Persistent polyclonal lymphocytosis has been described in a group of female patients who all have the HLA-DR7 antigen in common and who are all heavy cigarette smokers. Immunoglobulin heavy chain gene rearrangement was analyzed by hybridization with specific immunoglobulin heavy chain genes to restriction enzyme-diges...

journal_title：American journal of respiratory cell and molecular biology

authors： Chan MA,Benedict SH,Carstairs KC,Francombe WH,Gelfand EW

更新日期：1990-06-01 00:00:00

abstract：:An understanding of the molecular pathogenesis of lung cancer has evolved from classic cytogenetic studies and the use of restriction fragment length polymorphisms to encompass the definition of specific genetic abnormalities associated with this disease. Activation of the dominant class of oncogenes is frequent, with...

journal_title：American journal of respiratory cell and molecular biology

authors： Viallet J,Minna JD

更新日期：1990-03-01 00:00:00

abstract：:The effects of surfactant, surfactant lipids, and surfactant protein A (SP-A) on the surface phagocytosis of [3H]thymidine-labeled Staphylococcus aureus (SAE) by rat alveolar macrophages were studied. Alveolar macrophages only ingest SAE when the bacteria are opsonized with rat serum prior to incubation with alveolar ...

journal_title：American journal of respiratory cell and molecular biology

authors： van Iwaarden F,Welmers B,Verhoef J,Haagsman HP,van Golde LM

更新日期：1990-01-01 00:00:00

abstract：:Neutrophils were found to demonstrate chemotactic responses to pepsinized human placental type IV collagen and its purified aminoterminal 7S domain. The maximal chemotactic responses occur at approximately 400 ng/ml and approximately 30 ng/ml of type IV collagen and 7S collagen, respectively, and are similar in magnit...

journal_title：American journal of respiratory cell and molecular biology

authors： Senior RM,Hinek A,Griffin GL,Pipoly DJ,Crouch EC,Mecham RP

更新日期：1989-12-01 00:00:00